Improved Tissue Culture Conditions for Engineered Skeletal Muscle Sheets
نویسندگان
چکیده
منابع مشابه
Improved Tissue Culture Conditions for Engineered Skeletal Muscle Sheets
The potential clinical utility of engineered muscle is currently restricted by limited in vitro capacity of expanded muscle precursor cells to fuse and form mature myofibers. The purpose of this study was to use isotropic skeletal muscle sheets to explore the impact of (1) fibroblast coculture and (2) fibroblast-conditioned media (fCM) on in vitro myogenesis. Muscle sheets were prepared by seed...
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Skeletal muscle injuries are common in athletes, occurring with direct and indirect mechanisms and marked residual effects, such as severe long-term pain and physical disability. Current therapy consists of conservative management including RICE protocol (rest, ice, compression and elevation), nonsteroidal anti-inflammatory drugs, and intramuscular corticosteroids. However, current management o...
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OBJECTIVE Peripheral arterial disease (PAD) can have severe consequences on patient mortality and morbidity. In contrast to approaches using growth factor administration or isolated cell transplantation, we attempted to develop an alternative method for ischemic therapy using the transplantation of tissue engineered cell sheets with angiogenic potential. METHODS AND RESULTS Human smooth muscl...
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Human bioartificial muscles (HBAMs) are tissue engineered by suspending muscle cells in collagen/MATRIGEL, casting in a silicone mold containing end attachment sites, and allowing the cells to differentiate for 8 to 16 days. The resulting HBAMs are representative of skeletal muscle in that they contain parallel arrays of postmitotic myofibers; however, they differ in many other morphological ch...
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INTRODUCTION Engineered tissues offer strong possibilities as model systems for studying tissue responses to physical, chemical or biological stimuli. Moreover, they can be used to investigate specific pathologies or clinical treatments with less ethical considerations and better experimental control than animal models or human studies. We use the concept of tissue engineering to design in-vitr...
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ژورنال
عنوان ژورنال: The Scientific World Journal
سال: 2013
ISSN: 1537-744X
DOI: 10.1155/2013/370151